Optimizing the feeding operation of recombinant Escherichia coli during fed-batch cultivation based on Pontryagin's minimum principle

  • W Jin
  • D Fan
  • Y Luo
  • L Chi
  • JQ Guo
  • GP Zhang
  • C Zhu
Keywords: Fed-batch culture, human-like collagen, maximum specific growth rate, Pontryagin's minimum principle, recombinant Escherichia coli.

Abstract

Recombinant Escherichia coli BL21 was used to produce human-like collagen in fed-batch culture. After building and analyzing the kinetic models of fed-batch cultures, the maximum specific growth rate, Yx/s and Yp/s were 0.411 h-1 , 0.428 g·g-1 and 0.0716 g/g, respectively. The square error of cell growth models, glucose consumption model and human-like collagen formation were almost all around 94%, which indicated that the kinetic model could describe the actual change well. According to the target, that is, to gain the highest productivity of human-like collagen, the feeding rate (F) was worked out on the basis of Pontryagin's minimum principle. In the verification experiments, the specific growth rate was controlled at 0.15 and 0.04 h-1 at the fed-batch and induction phase, respectively. The result showed that the concentrations of cell and human-like collagen could reach 87.6 and 6.11 g·L-1, and they were raised by 17.9 and 18.6%, respectively.

Key words: Fed-batch culture, human-like collagen, maximum specific growth rate, Pontryagin's minimum principle, recombinant Escherichia coli.

Published
2013-11-13
Section
Articles

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eISSN: 1684-5315