Isolates from wound infections at federal medical centre, bida.
A total of 589 wound swabs from 334 patients in Federal Medical Centre, Bida were studied. Samples were collected between Jan 2002 to Dec. 2003. Swabs were plated within one hour after collection unto blood, chocolate and Mac Conkey after plate, and incubated aerobically for 24hrs. The chocolate plated swabs were incubated under increased carbon dioxide for 24hrs. Organism were identified using morphological and biochemical characteristics according to Cowon and Steel\'s manual for identification of medical bacterial. Organism isolated were subjected to antibiotic susceptibility testing by disc diffusion using modified Kirby-Bauer method. The number of swabs that were culture positives were 441 (74.9%). Out of these 441 samples 334 (75.7%) grew one organism each, 99 samples (22.4%) grew two organisms each while 8 samples (1.8%) grew three organisms each. The 3 commonest organisms isolated were Staphylococcus aureus (45.5%), Escherichia coli (21.8%), Pseudomonas aeruginosa (14.9%) which together constitute 82.2% of the isolates. There is a high level of antibiotic resistance. Ciprofloxacin is the drug of choice for the gram positive bacteria with susceptibility of 68.3%. Ofloxacin is the drug of the choice for the gram negative bacteria with susceptibility of 76.3%
African Journal of Clinical and Experimental Microbiology Vol. 9 (1) 2008: pp. 26-32