Anti-Tumor Effect of Cactus Polysaccharides on Lung Squamous Carcinoma Cells (SK-MES-1)

Abstract

Background: Cactus polysaccharides are the active components of Opuntia dillenii which have been used extensively in folk medicine. In this
study, we investigate the anti-tumor effect of cactus polysaccharides on lung squamous carcinoma cells SK-MES-1.
Materials and Methods: The inhibitory effect of Cactus polysaccharides on lung squamous carcinoma cells were detected by MTT assay. Cell
cycle was determined by flow cytometry and cell apoptosis was determined by AnnexinV assay. Western-blotting was applied to detect P53 and
PTEN protein expression in the cells treated with cactus polysaccharides.
Results: Results showed that different concentrations of wild cactus polysaccharides prevent SK-MES-1 cells growth and induces S phase arrest.
The data also revealed that cactus polysaccharides cause apoptosis in SK-MES-1 cells determined by Annexin-V assay. Furthermore, cactus
polysaccharides induced growth arrest and apoptosis may be due to the increase of P53 and phosphatase and tension homolog deleted on
chromosome ten (PTEN) protein.
Conclusion: Cactus polysaccharides have anti-tumor activity on lung squamous carcinoma cells.

Key words: Cactus polysaccharides, Lung squamous carcinoma, Anti-tumor effect, P53, PTEN

Abbreviations: PTEN :phosphatase and tension homolog deleted on chromosome ten; NSCLC: Non-small-cell lung cancer; FBS :Phosphate buffered saline; MTT:3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PBS: Phosphate buffered saline; DMSO:Dimethyl sulfoxide; PI: Propidium iodide.