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Modulatory effect of <i>Periostracum cicadae</i> and <i>Betulae</i> cortex extracts on the activation of atopic dermatitis-related ion channels orai1 and TRPV3

Joo Hyun Nama
Hyo Won Jung
Woo Kyung Kim
Hyo Sang Bae


Background: The cast-off shells of Cryptotympana pustulata (Periostracum Cicadae, PC) and the bark of Betula platyphylla (Betulae Cortex, BC) are used as traditional medicines for the treatment of skin diseases. This study was conducted to investigate the regulatory effects of PC and BC extracts on the activation of the ion channels, calcium release-activated calcium channel protein 1 (ORAI1) and transient receptor potential cation channel subfamily V member 3 (TRPV3).
Materials and Methods: Human HEK293T cells, co-overexpressing ORAI1/stromal interaction molecule 1 (STIM1) or overexpressing TRPV3, were treated with PC or BC extracts at 0.1 mg/mL. The changes in ORAI1 and TRPV3 activities were measured using a conventional whole-cell patch-clamp technique.
Results: PC and BC extracts significantly decreased ORAI1 activation in  ORAI1-STIM1 co-overexpressing HEK293T cells and significantly increased TRPV3 activation in TRPV3 overexpressing cells, compared to that of 2- aminoethoxydiphenyl borate (2-APB, 100 μM), a known agonist of TRPV3.
Conclusion: Our results suggest that PC and BC extracts have therapeutic potential to improve skin barrier abnormalities in atopic dermatitis via modulation of ORAI1 and TRPV3 activation.

Keywords: Cryptotympana pustulata; Betula platyphylla var. japonica; calcium  channels; modulatory effect Abbreviations: AD, atopic dermatitis; BAPTA,  1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid; BC, Betulae Cortex; LTC4, leukotriene C4; NFAT, nuclear factor of activated T cells; ORAI-1, calcium release-activated calcium channel protein 1; PC, Periostracum Cicadae; TG, transglutaminase; TRPV3, transient receptor potential cation channel subfamily V member 3; TSLP, thymic stromal lymphopoietin; 2-APB, 2-aminoethoxydiphenyl borate; STIM1, stromal interaction molecule 1; IP3, inositol 1,4,5-trisphosphate.

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eISSN: 0189-6016