Effects of stem-bark extract of Okoubaka aubrevillie on some visceral organs of Wistar rats

  • Peter U Achukwu
  • Silas A. Ufelle
  • Kenechukwu C. Onyekwelu
  • Millicent N. Amadi
  • Ngozika O. Achukwu
  • Francis N. Amadi
Keywords: Acute toxicity, Graded doses, Mortality, Sub-acute, Toxin inhibition.

Abstract

Background: Over the past two decades, there has been a tremendous  increase in the use of herbal medicine; however, these herbs have not  been properly evaluated to ascertain their effect on the body organs.
Materials and Methods: Effects of stem bark extract of Okoubaka  aubrevillie on some visceral organs were investigated in Wistar rats. For acute toxicity testing, Wistar rats (n=16), grouped into 4, (A-D) orally  received graded doses of Okoubaka aubrevillie extract and deaths recorded within 24 hours. For sub-acute study, Wistar rats (n=20) grouped into 5,  (A-E) orally received graded doses of Okoubaka aubrevillie extract for 31 days. Blood samples were collected from each rat through retro-orbital  puncture for biochemical analysis. The liver, kidney and stomach were  excised and processed for light microscopy. For toxin inhibition studies, Wistar rats (n=24) grouped into 6 (A-F), were used. Groups A-C and D-F orally received graded doses of Dichlorvos. Groups A-C further received  Okoubaka aubrevillie extract while D-F received water and death records observed.
Results: For acute toxicity testing, lethal dose (LD50) of 7500 mg/kg body weight was obtained from the inverse of the log-dose. Sub-acute studies revealed significantly elevated mean body weight in group A (210 ± 4.5 gram) compared to control (178 ± 5.0 gram), (p<0.05). Liver of rats in group A revealed some areas of moderate peri-portal lymphatic inflammation. Treated groups revealed intact architecture of liver, kidney and stomach.
Conclusion: Okoubaka aubrevillie extract was found to be relatively safe for consumption and is capable of inhibiting toxins.


Key word: Acute toxicity; Graded doses; Mortality; Sub-acute; Toxin inhibition.

Published
2018-06-05
Section
Articles

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eISSN: 0189-6016