Slurry nebulization ICP-OES for the determination of Cu, Fe, Mg, Mn and Zn

  • Ntebogeng S. Mokgalaka Tshwane University of Technology, Department of Chemistry, Arcadia Campus, P.O. Box 56208, Arcadia 0007, South Africa
  • Taddese Wondimu National University of Lesotho, Department of Chemistry and Chemical Technology, P.O. Roma 180, Lesotho
  • Robert I. McCrindle Tshwane University of Technology, Department of Chemistry, Arcadia Campus, P.O. Box 56208, Arcadia 0007, South Africa
Keywords: Bovine liver, ICP-OES, Slurry nebulization, Cu, Fe, Mg, Mn, Zn

Abstract

A method that involves analysis of bovine liver by slurry nebulization and ICP-OES has been developed. This method permits rapid and accurate determination of Cu, Fe, Mg, Mn and Zn in bovine liver. Aliquots of freeze-dried and powdered bovine liver sample were dispersed in 2.0 M HNO3 and sonicated to homogenize the resulting slurries. Bovine liver samples were also microwave digested or subjected to aqueous extraction for comparison of analytical results. Concentrations of Cu, Fe, Mg, Mn and Zn in aqueous slurries, the digests, and aqueous extracts were determined by the ICP-OES using external calibration curves. A student’s t-test showed that the results obtained using the slurry method were in good agreement at 95 % confidence level (CL) with those of microwave digestion or aqueous extraction techniques, except for Fe. To check the accuracy and precision of the slurry method, a bovine liver CRM was analyzed and good agreement was achieved with the certified values at 95 % CL. The results demonstrate inefficiency of aqueous extraction technique for complete removal of Fe in bovine liver sample.

 

KEY WORDS: Bovine liver, ICP-OES, Slurry nebulization, Cu, Fe, Mg, Mn, Zn

 

 

Bull. Chem. Soc. Ethiop. 2008, 22(3), 313-321.

 

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eISSN: 1726-801X
print ISSN: 1011-3924