Bulletin of the Chemical Society of Ethiopia

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Interaction mode between methylene blue-Sm(III) complex and herring sperm DNA

Y Hu, X Wang, H Pan, L Ding


Spectroscopic and viscosity methods were applied to investigate the interaction between methylene blue (MB)-Sm(III) complex and herring sperm DNA by using acridine orange as a spectral probe in Tris-HCl buffer (pH 7.40). By means of molar ratio method, the binding ratios between MB-Sm(III)and DNA were determined as nSm(III):nMB =1:3, nMB-Sm(III) complex:n DNA = 8:1, and the apparent molar absorption coefficient of εMB-Sm(III)-DNA was found to be 7.01×105 M-1·cm-1. The bonding constants at different temperatures Kθ292K = 7.35×105 M-1, Kθ310K = 1.25×105 M-1 were obtained by double reciprocal method. Thermodynamic function computation demonstrates that ΔrSmӨ is the primary driving power of the interaction between MB-Sm(III)and herring sperm DNA. By combination analysis of the Scatchard method, circular dichroism spectroscopy and viscosity method, it suggests that the interaction mode between MB-Sm(III) complex and herring sperm DNA are a mixed binding, which contains partial intercalation and electrostatic interaction.


KEY WORDS: Methylene blue-Sm(III), Complex, Herring sperm DNA, Interaction


Bull. Chem. Soc. Ethiop. 2012, 26(3), 395-405.

AJOL African Journals Online