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Egyptian Journal of Biochemistry and Molecular Biology

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Lipoprotein Lipase mRNA expression in different tissues of farm animals

AM Elkattawy, SA El-Shazly, KA KAhilo, IF Hassan

Abstract


Lipoprotein lipase (LPL) controls triacylglycerol partitioning between
adipose tissues and muscles, so it is important enzyme for fattening of
animals .The present work was planned to clarify the use of
polymerase chain reaction (PCR) for detection of LPL mRNA
expression in different tissues representing internal organs of male
Friesian cows. The obtained results revealed that, LPL mRNA was
expressed in all tested tissues, and the highest expression was
encountered in testis (1.03). The expression in heart, liver, kidney,
adrenal gland, spleen were 0.92, 0.77, 0.76, 0.73, and 0.70
respectively .The lowest expression was found in adipose tissue (0.64)
It could be concluded from the present study that, expression of LPL
mRNA differs according to the type of tissue. The highest expression
was found in testis which may confirm the role of LPL in the process
of spermatogenesis.



http://dx.doi.org/10.4314/ejbmb.v27i1.43191
AJOL African Journals Online