PROMOTING ACCESS TO AFRICAN RESEARCH

Egyptian Journal of Biochemistry and Molecular Biology

Log in or Register to get access to full text downloads.

Remember me or Register



DOWNLOAD FULL TEXT Open Access  DOWNLOAD FULL TEXT Subscription or Fee Access

Mitochondrial pathway of apoptosis and related proteins in placenta of pregnant Egyptian women with pre-eclampsia

MM Sallam, RM Sallam, NM El Kholy, A Alyan

Abstract


Apoptosis within the placenta is increased in pregnancies complicated by pre-eclampsia (PE).This study aimed at evaluating the mitochondrial pathway of apoptosis in placenta of pregnant women with pre-eclampsia and correlate it with severity and pregnancy outcome . Apoptosis was assessed by measuring DNA fragmentation%,Bcl-2, p53 and caspase-9 in placental tissues from 25 pregnancies complicated by pre-eclampsia, and 25 placentas of normal pregnancy (NP). DNA fragmentation, p53 and caspase-9, were significantly increased while, Bcl-2 was significantly decreased in the placenta of pre-eclampsia group compared to control. No significant difference between mild and severe pre-eclampsia subgroups
regarding these parameters could be found. DNA fragmentation was negatively correlated with Bcl-2 in PE group. Moreover, DNA fragmentation was negatively correlated with maternal age in both PE and control groups. A significant positive correlation between placental DNA fragmentation and gestational age in the NP group was observed. A significant negative correlation between caspase-9 activity, and fetal weight in PE group was found. It can be concluded that Hypoxia and ischemia induced by pre-eclampsia, up regulate p53 and reduce Bcl-2 expression with activation of caspase-9 and increasing DNA fragmentation in placental tissue. These results implicate the mitochondrial pathway in enhancing apoptosis in preeclamptic placenta and affecting fetal outcome but not the severity.

Keywords : Apoptosis, p53, Bcl-2, Caspase-9, Mitochondria,
Pre-eclampsia.




http://dx.doi.org/10.4314/ejbmb.v28i1.54365
AJOL African Journals Online