Study of some biochemical and genetic risk factors for atherosclerosis in systemic luus erythematosus patients

  • MM El-Batch
  • SS Zakarial
  • H Mourad
  • H El Saadany
  • T Moustafa

Abstract

The aim of this study was to evaluate the relation between asymmetric dimethylarginine (ADMA ), high sensitive C-reactive protein (hs-CRP), monocyte chemoattractant protein-1 (MCP-1) ( both serum levels and the genotypes of the MCP-1 A-2518G polymorphism) with the development of carotid atherosclerosis in systemic lupus erythematosus patients (SLE). Thirty non menopause SLE female patients and twenty healthy age-matched females were included. Both cases and controls were subjected to evaluation of body mass index (BMI), intimal-medial thickness (IMT), fasting blood sugar (FBS), serum lipids. Serum ADMA, hs-CRP, and MCP-1, levels were measured by ELISA. MCP-1 polymorphism was detected by PCR-RFLP. Our results showed that values for IMT, hs-CRP, ADMA and MCP-1, were significantly higher in SLE patients than in
healthy control with more significant increase in SLE patients with IMT <1 mm than those with IMT < 1 mm. Carotid IMT was significantly positively correlated with all the studied variables except for age, BMI and FBS, but significantly negatively correlated with HDL-C in all SLE patients. Genotype of MCP-1 (A-2518G) showed that; G/G genotype was more frequent in SLE patients than controls. IMT, hs- CRP, ADMA and MCP-1 from patients with G/G phenotypes were markedly higher than patients with the A/A or A/G
genotype. In multiple regression analysis, ADMA and MCP-1 were the strongest independent determinants of IMT in SLE patients. In conclusion assessment of high levels of ADMA, hs-CRP, MPC-1, in addition to the MCP-1 G allele may play a role in the pathogenesis of accelerated atherosclerosis in SLE patients and would be useful in identifying the risk of developing atherosclerosis.

Keywords: asymmetric dimethylarginine (ADMA ), gene polymorphism, high sensitive C-reactive protein (hs-CRP), intimal media thickness (IMT), monocyte chemoattractant protein-1 (MCP-1), systemic lupus erythematosus (SLE)

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