Adiponectin Single Nucleotide Polymorphism (+276G/T) and Its Possible Relation to Adiponectin Level in Egyptian Patients with Coronary Artery Disease
Increasing interest has been directed toward the role of the adiponectin gene polymorphism in the human genome and its implication in the pathogenesis of coronary artery disease. The present study was investigating the association between the single nucleotide polymorphism +276 G/T of the adiponectin gene with serum adiponectin level in patients with coronary artery disease (CAD). In this study 100 healthy controls and 100 Egyptian patients with coronary artery disease of both genders presented to the Cardiology Department of Suez Canal University Hospital were investigated. All subjects were genotyped for +276 G/T polymorphism of adiponectin gene. Lipid profile, fasting blood glucose were measured. Adiponectin and high sensitivity C-reactive protein levels were determined by ELISA technique. Polymerase chain reaction based on restriction fragment length polymorphism (PCR-RFLP) was used to determine the genotypes of the studied population. The lowest serum adiponectin value was observed in patients with CAD compared with control group. The T allele of SNP +276 G/T in the adiponectin gene was found to be associated with CAD (odd ratio 2.23; 95% CI: 1.44-3.45; P= 0.001). The significant
association of the T allele (GT+TT) of this SNP with lower adiponectin level and higher hsCRP levels was confirmed in the study (p= 0.003 and 0.006 respectively). Our results concluded that, +276 G/T SNP in the adiponectin gene is associated with CAD. Furthermore, carriers of the at-risk T allele had lower serum adiponectin level and higher serum hsCRP, causing in turn an increased risk to develop CAD.
Key Words: Adiponectin gene; polymorphism; Coronary artery disease; PCR-RFLP