PROMOTING ACCESS TO AFRICAN RESEARCH

Global Journal of Pure and Applied Sciences

Log in or Register to get access to full text downloads.

Remember me or Register



DOWNLOAD FULL TEXT Open Access  DOWNLOAD FULL TEXT Subscription or Fee Access

Modifying and adapting a plant-based DNA extraction protocol for human genomic DNA extraction: a cost effective approach

M.E. Kooffreh, O.U. Udensi, A.J. Umoeyen

Abstract


This research involved modification of a standard protocol for DNA extraction in plants for its suitability as an extraction method for DNA in human whole blood in which some of the plasma was removed. Human blood samples were obtained from a 100 apparently healthy individuals residing in Calabar. The modified DNA procedure yielded good quality genomic DNA which was used in carrying out allele specific polymerase chain reaction which also yielded good quality amplicons. This method is simple and suitable for the extraction of DNA from human red cell.

Keywords: Genomic DNA, Human red blood cells, amplicons




http://dx.doi.org/10.4314/gjpas.v23i1.1
AJOL African Journals Online