Antioxidant Properties and In-Vitro Radical Scavenging Activities of Tannin-Rich and Flavonoid-Rich Fraction of <i>Annona senegelensis</i> and <i>Vernonia amygdalina</i> leaves

Abstract

Medicinal Plants have demonstrated history of managing some ailments caused by free radicals as a result of some chemical constituents they possess. This study was aimed at assessing antioxidant and free radical scavenging properties of Tannin-rich and Flavoniod-rich fraction of Annona senegelensis and Vernonia amygdalina leaves via in vitro assays such as; reducing power, nitric oxide scavenging activity, Hydrogen peroxide scavenging activity and Lipid peroxidation scavenging activity. The results obtained indicated that both medicinal plants are antioxidant reservoir. The values for Tannin rich fraction of Annona senegelensis (TRFAS) and Tannin rich fraction of Vernonia amygdalina (TRFVA) are Reducing power absorbance TRFAS (0.077-0.187), TRFVA (0.168-0239). % Nitric oxide scavenging: TRFAS (4.46-30.40), TRFVA (5.23-42.24). % H2O2 scavenging: TRFAS (7.30-20.35), TRFVA (8.12-22.32). % Lipid Perioxidation: TRFAS (6.81-32.76), TRFVA (5.16-26.16). Also the values for Flavonoid rich fraction of Annona senegelensis (FRFAS) and Flavonoid rich fraction of Vernonia amygdalina (FRFVA) are reducing power absorbance: FRFAS (0.109-0.342), FRFVA (0.124-0.388). % Nitric oxide scavenging: FRFAS (33.55-43.57), FRFVA (21.10-47.46). % H2O2 scavenging: FRFAS (39.01-74.96), FRFVA (45.80-75.20). % Lipid Perioxidation: FRFAS (24.81-59.69), FRFVA (41.43-59.98). The Tannin-rich and Flavonoid-rich fraction of both plants exhibited good antioxidant activity on all models employed at increasing concentrations but Flavoniod-rich fraction of Vernonia amygdalina had the highest inhibitory effect than the Tannin fraction. The findings from this study validated the pharmacological potency of the two plants and their potential use in combating free radical-related diseases, which are often triggered by oxidative stress.