Fine mapping of trypanosomosis resistance loci in murine by haplotype approach

Abstract

Trypanotolerance QTL has been mapped in two F2 mouse populations developed by crossing susceptible strains, A/J and BALB/c, with the resistant C57BL/6J. Identifying regions of shared haplotype in inbred lines known to harbour quantitative trait loci for disease resistance may assist to fine map the QTL locus. The 129/J mouse strain is susceptible to trypanosomosis but it is not known if it carries susceptible alleles at the previously mapped QTL. We undertook to map QTL in 138 F2 mice from 129/J x C57BL/6J crossings by selective genotyping following challenge with T. congolense. Chromosome 1 and 17 were confirmed to carry the susceptible QTL alleles previously identified in A/J and C57BL/6J strains. Chromosomes 2, 3, 5 and 15 did not show a significant QTL. Further analysis using the International SNP database suggested the presence of a shared haplotype which if confirmed, should reduce the confidence interval of the QTL on chromosome 1and 17.

Kenya Veterinarian Vol. 31 (2) 2007: pp. 64-67