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Background: The aim of the study was to evaluate the antimicrobial activity of the‑potassium‑titanyl‑phosphate ‑ the KTP laser and ozone in of primary root canals.
Materials and Methods: Sixty primary incisor teeth were selected. The specimens were inoculated with 10 μL Enterococcus faecalis (E. faecalis). Groups: The KTP laser (1,5 W); gaseous ozone (150 s); sodium hypochlorite (NaOCl); saline group. Sterile paper points used to sample bacteria from the canals to tubes containing 5 mL of brain heart infusion broth. Then, 10 μL suspension was incubated in culture media for 24 h. Data were analyzed statistically using Kruskal–Wallis and Mann–Whitney U‑test.
Results: There were statistically significant differences between all groups (P < 0.05). Complete sterilization was achieved in the 2.5% NaOCl group. The number of bacteria were significantly reduced in experimental groups in comparison to the saline group.
Conclusion: The KTP laser and ozone application provided a significant antibacterial effect in primary root canals; however, 2.5% NaOCl was superior.
Keywords: Antibacterial activity, KTP laser, ozone, primary root canals