<i>Arachis hypogaea</i> seed powder ameliorated 1, 2-dimethylhydrazine-induced oxidative stress in exposed rats

Abstract

This study evaluated the effect of Arachis hypogaea seeds on 1, 2 – dimethylhydrazine-induced oxidative stress in the colon and liver of male and female rats. Eighty-four rats of both sexes were used for this study and were divided into seven groups of 6 rats each. 1, 2- dimethylhydrazine (DMH) was administered subcutaneously at a dose of 25 mg/kg body weight. Group A (control) rats of both sexes were maintained on normal rat feed. The Group B rats were maintained on normal feed and administered DMH once weekly for 12 weeks. The Group C rats were provided normal feed and administered DMH weekly for 24 weeks. Group D rats were administered DMH and normal feed for 12 weeks followed by a peanut-supplemented diet for the next 12 weeks. The Group E rats received DMH weekly and peanut-supplemented diet concomitantly for 24 weeks. Group F rats had a peanut-supplemented diet for 12 weeks before DMH administration for another 12 weeks. Group G rats were maintained only on a peanut-supplemented diet for 24 weeks. The result shows that DMH significantly (p ≤ 0.05) increased malondialdehyde level in the colon but the increase was reversed by the incorporation of peanut in the diet. There were significant (p ≤ 0.05) decreases in antioxidant enzyme activities and reduced glutathione levels in the groups that received DMH alone which were reversed in the group of rats exposed to DMH and peanut-supplemented diet. The findings indicate that the consumption of peanut-supplemented diet reduced in rats, the increase in lipid peroxidation occasioned by DMH.