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Tropical Journal of Pharmaceutical Research

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Suppression of long non-coding RNA H19 inhibits proliferation, cell migration and invasion in human cervical cancer cells

Huawei Xin, Mingzhe Li, Xiaoling Cheng, Tao Wang, Xiaoliu Liu, Yan Zhang

Abstract


Purpose: To determine the expression profile of lncRNA H19 in different cervical cancers, and to decipher its function in the growth and metastasis of cervical cancer.
Methods: The analysis LncRNA H19 expression was performed using quantitative real time
polymerase chain reaction (qRT-PCR). Cell counting kit 8 (CCK8) assay was used to assess the viability of the cells. The cells were transfected with Si-H19 using Lipofectamine 2000 and the metastasis of cells was determined by cell migration and invasion assay. Immunoblotting was used to evaluate the protein expression.
Results: The lncRNA H19 expression was considerably enhanced in cervical cancer cells, and was about 2.6 to 5.3 times more in cervical cancer cells relative to non-cancer cells. Inhibition of lncRNA caused significant reduction in cervical cancer cell growth in a time-dependent manner. In addition while silencing of lncRNA inhibited the metastasis of HeLa cells. Cell migration and invasion was about 26 % in Si-H19 transfected cervical cancer cells, relative to 65 % in Si-NC cervical HeLa cells. Similarly, cell invasion was 45 % in Si-H19 cervical HeLa cells relative to the negative control (Si-NC). Inhibition of HeLa cell metastasis was also concomitant with decline of metalloproteinases (MMP)-2 and 9
expression.
Conclusion: lncRNA regulates the growth and metastasis of cervical cancer cells. Thus, IncRNA may be an important therapeutic agent for cervical cancer.

Keywords: Cervical cancer, lncRNA, Proliferation, Invasion




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