Electrospray ionization-mass spectrometry of different extracts of the organs of Rumex cyprius and their antihepatotoxic effect

  • Basma G Eid
  • Nadia M Sokkar
Keywords: Rumex cyprius, Mass spectrometry, HPLC, Antihepatotoxicity


Purpose: Phytochemical and biological investigations of the valuable plant, Rumex cyprius, family Polygonaceae, wildly grown in Saudi Arabia.
Methods: Chloroform, ethyl acetate and methanol extracts were prepared from the different organs of R. cyprius. The extracts were analyzed by electrospray ionization source coupled to a mass spectrometer (ESI-MS) and tandem mass spectrometer (ESI-MS/MS) at different collision energies. The plant organs (leaf, fruit and stem) were standardized on the bases of quercetin by HPLC, and determined for their hepatoprotection in tetrachloride-induced acute liver toxicity using a mouse model.
Results: Twenty-five phenolic compounds distributed between the leaf, fruit and stem of R. cyprius were identified. They were related to classes of anthraquinones, phenolic acids, flavonoid aglycones, glycosides and polyphenols. Twenty-two compounds in total were found and identified, and for the hepatoprotective effects, the leaf exhibited the best activity.
Conclusion: R. cyprius is a source of potentially active phytoconstituents and a good natural
hepatoprotective drug. This study is being documented for the first time.


Journal Identifiers

eISSN: 1596-9827
print ISSN: 1596-5996