Antimicrobial Activity of the Leaves of Endemic Stachys pseudopinardii in Turkey
AbstractPurpose: The ethanol extract of the leaves of Stachys pseudopinardii R. Bhattacharjee and Hub.–Mor. (Lamiaceae) were investigated for their antimicrobial activities.
Methods: The antimicrobial activity of the leaf extract of the plant was tested against Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 7064, Staphylococcus aureus ATCC 6538P, Escherichia coli ATCC 10538, Proteus vulgaris ATCC 6899, Salmonella typhimurium CCM 5445 and Pseudomonas aeruginosa ATCC 27853, as well as Candida albicans ATCC 10239, Debaryomyces hansenii DSM 70238, Kluyveromyces fragilis ATCC 8608 and Rhodotorula rubra DSM 70403, by disc diffusion and microdilution methods. Selected antibacterial agents (penicillin, tobramycin and ampicillin) and antifungal agents (nystatin, clotrimazole and ketoconazole) antibiotics were used as positive reference standards in the tests.
Results: The extracts showed strong antibacterial activity against Bacillus cereus ATCC 7064, with an inhibition zone of 25.0 mm, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 16 and 32 μg/mL, respectively. Debaryomyces hansenii DSM 70238 was among the most susceptible of the yeast cultures, with an inhibition zone of 17.0 mm and MIC and minimum fungicidal concentration (MFC) of 32 and 32 μg/mL, respectively. The extract exhibited moderate activity against the other test microorganisms.
Conclusion: The results demonstrate that the ethanol extract of the leaves of Stachys pseudopinardii has significant antimicrobial activity and suggest that it may be useful in the treatment of infections.
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.