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<i>In vitro</i> multiplication of banana (Musa sp.) cv. Grand Naine

S Ahmed
A Sharma
AK Singh
VK Wali
P Kumari


A micropropagation method is described for banana (Musa Spp.) Cv. Grand Naine. Suckers were surface sterilized with HgCl2 (0.1%) for 6 min which gave minimum contamination with maximum culture establishment. Of various treatment combinations, Murashige and Skoog (MS) medium + BAP 4.00 mg/l with IAA 2.00 mg/l resulted in maximum establishment of cultures in lesser time. MS medium + BAP 4.00 mg/l + IAA 2.00 mg/l gave maximum multiple shoots. Maximum rooting was obtained on MS medium (half strength) supplemented with IBA 1.00 mg/l and activated charcoal 200 mg/l.

Keywords: Grand naine, micro propagation, murashige and skoog medium, Musa spp. and suckers.

African Journal of Biotechnology, Vol 13(27) 2696-2703
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eISSN: 1684-5315