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African Journal of Biotechnology

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In vitro multiplication of banana (Musa sp.) cv. Grand Naine

S Ahmed, A Sharma, AK Singh, VK Wali, P Kumari

Abstract


A micropropagation method is described for banana (Musa Spp.) Cv. Grand Naine. Suckers were surface sterilized with HgCl2 (0.1%) for 6 min which gave minimum contamination with maximum culture establishment. Of various treatment combinations, Murashige and Skoog (MS) medium + BAP 4.00 mg/l with IAA 2.00 mg/l resulted in maximum establishment of cultures in lesser time. MS medium + BAP 4.00 mg/l + IAA 2.00 mg/l gave maximum multiple shoots. Maximum rooting was obtained on MS medium (half strength) supplemented with IBA 1.00 mg/l and activated charcoal 200 mg/l.

Keywords: Grand naine, micro propagation, murashige and skoog medium, Musa spp. and suckers.

African Journal of Biotechnology, Vol 13(27) 2696-2703



http://dx.doi.org/10.5897/AJB2014.13750
AJOL African Journals Online