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compounds were hydrolyzed by the resting cells. The main (cyano-)amide products demonstrated that nitrile hydratase was abundantly produced in this strain and that it mediated monohydrolysis. The specific conversion rate decreased in the following order: acrylonitrile > 3-cyanopyridine > valeronitrile
> adiponitrile > 2,3,4,5,6-pentafluorobenzonitrile > -hydroxyphenylacetonitrile > 3-indoleaceto-nitrile > phthalonitrile, suggesting a higher conversion capability towards aliphatic nitriles. The strain that had
broad substrate spectra was identified and named Rhodococcus ruber CGMCC3090 based on the 16S rDNA sequence.