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African Journal of Biotechnology

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pLIVE-EGFP: A liver specific vector carrying the EGFP reporter for transgenic screening and expression

S Zhang, X Ma, J Tang, S Liang

Abstract


pLIVE-EGFP is a liver-specific expression vector constructed by insertion of IRES-EGFP gene at the Xho I site of the pLIVE vector. The pLIVE-EGFP vector permits simultaneous expression of a gene of interest in addition to the EGFP reporter, specifically within liver cells, both in vivo and in vitro. When expressed in liver cells, the EGFP reporter produces a bright green fluorescence, which can easily be detected with fluorescence microscopy or flow cytometry. Compared with its parental vector, the pLIVE-EGFP is more
powerful in providing a simple, direct method for monitoring gene transfection efficiency, gene expression and for screening positive clones.



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