Main Article Content
involved in domestic animal diseases, including Escherichia coli, Salmonella pullorum, Pseudomonas aeruginosa, Pasteurella multocida, Clostridium perfringens, Micrococcus luteus, Streptococcus bovis
and Staphylococcus aureus. Two multidrug-resistant clinical isolates and a phytopathogenic yeast strain were also inhibited. The antimicrobial substance was secreted at the middle of the exponential phase, whose activity was sensitive to proteinase K and pronase E but resistant to the proteolytic action of papain, trypsin and pepsin. The antimicrobial activity was relatively heat resistant and also active over a wide range of pH 3 - 10. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
analysis revealed that the active peptide had an apparent molecular weight of about 6.3 kDa. It exhibited a bactericidal activity against S. aureus IVDC C56005. Such characteristics indicate that this bacteriocin may be a potential candidate for alternative agents to control important pathogens in domestic animal diseases.