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Main Article Content
Purification and Some Properties of Rubber Seed Lipoxygenase
Abstract
Lipoxygenase was extracted from rubber seed and purified by ammonium sulphate precipitation, gel filtration on Sephadex G – 25 and ion – exchange chromatographies on DEAE – cellulose columns. The enzyme was purified 78 fold and 38.56% of the enzyme activity was recovered in the purification. The molecular weight of the enzyme was 102,000 daltons and there were two subunits of the enzyme with molecular weights of 49,000 and 107,000 daltons. The rubber seed lipoxygenase had two ionizing groups at the pK values of 4.8 and 7.3. It also had pK values of 5.2 and 7.5 as the ionization constants of the free enzyme. The activation energy (Ea) of the rubber seed lipoxygenase was 7.2 kcal/mole and the enthalpy (DH) was 5.3 kcal/mole.
Key Words: Lipoxygenase, Rubber See
Bio-Research Vol.1(2) 2003: 21-31
Key Words: Lipoxygenase, Rubber See
Bio-Research Vol.1(2) 2003: 21-31
