Purification and Some Properties of Rubber Seed Lipoxygenase

  • MN Anokwulu Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Keywords: Lipoxygenase, Rubber See

Abstract

Lipoxygenase was extracted from rubber seed and purified by ammonium sulphate precipitation, gel filtration on Sephadex G – 25 and ion – exchange chromatographies on DEAE – cellulose columns. The enzyme was purified 78 fold and 38.56% of the enzyme activity was recovered in the purification. The molecular weight of the enzyme was 102,000 daltons and there were two subunits of the enzyme with molecular weights of 49,000 and 107,000 daltons. The rubber seed lipoxygenase had two ionizing groups at the pK values of 4.8 and 7.3. It also had pK values of 5.2 and 7.5 as the ionization constants of the free enzyme. The activation energy (Ea) of the rubber seed lipoxygenase was 7.2 kcal/mole and the enthalpy (DH) was 5.3 kcal/mole.

Key Words: Lipoxygenase, Rubber See

Bio-Research Vol.1(2) 2003: 21-31
Published
2004-04-28
Section
Articles

Journal Identifiers


eISSN: 2705-3822
print ISSN: 1596-7409