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Egyptian Journal of Medical Human Genetics

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Lower HOMA-β values are detected among individuals with variant of E23K polymorphism of potassium inwardly-rectifying channel, subfamily J, member 11 (KCNJ11) gene

R Sunita, AH Sadewa, A Farmawati

Abstract


Background: Type 2 Diabetes Mellitus (T2DM) is a multifactorial disease involving both genetic and also environmental factors. Potassium inwardly-rectifying channel, subfamily J, member 11 (KCNJ11) gene, an ATP-sensitive potassium channel-coding gene, contributes to insulin secretion.
Objectives: This research aimed to investigate E23K polymorphism in KCNJ11 gene and insulin secretion in individuals with family history of T2DM (cases) and without family history of T2DM (controls).
Method: This research was a case-control study involving 34 cases and 34 controls. E23K polymorphism of KCNJ11 was detected with PCR-RFLP. All of the obtained data were statistically analyzed with T-test, Mann–Whitney U-test, Chi-Square and One-Way ANOVA.
Result: Frequency of AA genotype in individuals with family history of T2DM (41%) was higher than in individuals without family history of T2DM (6%) (p=0.001). Frequency of A allele in individuals with family history of T2DM (68%) was higher than in individuals without family history of T2DM(38%) (p=0.001). The risk of A allele in individuals with family history of T2DMwas 3 times higher than in individuals without family history of T2DM (p=0.001, OR 3.38, CI 95% 1.67–6.84). Homeostasis Model Assessment b (HOMA-β) values of AA genotype (85.44%±39.55) were lower than that of GA (212.20%±79.30) and GG (254.00%±61.98) genotypes (p=0.000).
Conclusion: The risk of having A allele in individuals with family history of T2DM is higher than that in individuals without family history of T2DM. HOMA-β values of AA genotype are lower than that of GA and GG genotypes.




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