Cytochrome P450 2B6 6 distribution among Burkinabè population

Abstract

Mutations affect the genes that encode certain cytochromes P450 (CYP450) isoenzymes that are responsible for metabolism of drugs. The  aim of this study was to determine the frequency of CYP2B6 (516G>T) SNP in the Burkinabè population. Genomic DNA extraction  from blood was performed by GenJET® Genomic DNA according to the manufacturer’s instructions. Genotyping for the cytochrome P450  variant alleles was performed using predesigned primers. The amplification was carried out by PCR while the differentiation between the  alleles was carried out after digestion with restriction enzymes by electrophoresis. The CYP2B6 c.516G>T was successfully analyzed in  92,38% (291/315) of the study participants. Among the investigated individuals, the distribution of the major allele CYP2B6*G was 56%  and the minor CYP2B6*T allele was 44%. The results obtained showed a prevalence of 56% for the G allele and 44% for the T allele. This  was distributed as 31% for the GG genotype (reduced dosage required), 51% for the GT genotype (normal dosage range), and 18% for the  TT genotype (high drug toxicity). The presence of the rapid and poor metabolizer phenotypes in the studied sample shows the need  for additional studies to better assess their impact on Burkinabe people.