Article Sidebar
Article Details
Issue
Section
Articles
Submission of a paper for publication implies the transfer of the copyright from the author(s) to the publisher upon acceptance. International Formulae Group is therefore the copyright holder after publication of an article in International Journal of Biological and Chemical Sciences, and published articles should not be used for commercial purpose without the written consent of the Editor-in-Chief. They are licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License.
Main Article Content
Micropropagation of <i>Sphaeranthus amaranthoides</i> Burm. f. – A multipurpose medicinal herb
Abstract
A method for induction of multiple shoots using axillary bud and shoot tips of Sphaeranthus amaranthoides Burm. f. was described. The experiment was conducted in which multiple shoot induction was noticed on MS (Murashige and Skoog) medium supplemented with different concentrations of two cytokinins (BAP and Kin). These multiple shoots later developed into normal shoots. The highest rate of shoot proliferation was found to be 90% and 75% for axillary bud and shoot tip explants, respectively, on the
medium containing BAP 4.0 mg/l. The regenerated shoots were successfully rooted on MS medium supplemented with 2.0 mg/l IBA, after sequential hardening, survival rate was 90%.
Keywords: In vitro, Axillary bud, Shoot tips, Multiple shoots, Regeneration, Sphaeranthus amaranthoides Burm. f.
International Journal of Biological and Chemical Sciences Vol. 2 (4) 2008: pp. 579-586
