Evaluation of the effect of co-administration of resveratrol and vitamin E on carbamazepine-induced oxidative stress in male adult wistar rats

Abstract

Background: Carbamazepine (CBZ) as a drug used in the treatment of epilepsy and neuropathic pain has been shown to stimulate the effects of free radicals. Resveratrol, known as 3,5,4′-trihydroxystilbene, is found in grapes and other plant product. It effectively scavenges free radicals and other oxidants. Vitamin E is a lipid soluble antioxidant present in all cellular membranes. The present study assessed the combined effect of vitamin E and resveratrol on biomakers of carbamazepine- induced oxidative stress. Methods: Adult male Wistar rats (n = 25) were randomly allotted to five groups:  Group I (control) received distilled water; Group II received CBZ (50 mg/kg); Group III received CBZ (50 mg/kg) and vitamin E (200 mg/kg); Group IV received CBZ (50 mg/kg) and resveratrol (20mg/kg); Group V received CBZ (50 mg/kg) and the co-administration of vitamin E at 200 mg/kg and resveratrol at 20 mg/kg. Administration was done orally daily for 45 days, after which the animals were sacrificed and sera samples were used for biochemical analyses. The results show that treatment with CBZ significantly (p<0.01) increased malondyaldehyde (MDA) serum level and decreased the levels of oxidative stress bio-makers [superoxide dismutase (SOD), catalase(CAT) and glutathione peroxidase (GPx)] when compared to that of normal control. However, treatment with vitamin E (200mg/kg) and resveratrol (20mg/kg) significantly (p<0.01) reduced CBZinduced increase in serum MDA level and increased the level of oxidative stress bio-makers (SOD, CAT and GPx) in comparison to CBZ-treated group. The co-administration of vitamin E (200mg/kg) and resveratrol (20mg/kg) showed non-statistically significant increase in SOD, CAT and GPx and reduced serum MDA level in comparison to either vitamin E or resveratrol treated group. The results support that vitamin E 200mg/kg and resveratrol 20mg/kg or their combination ameliorates CBZ-induced oxidative stress in male Wistar rats. The effects of these antioxidants are considered to be related to their intrinsic ability to scavenge free radicals