Processing, screening and microbiological characterization of microcrystalline cellulose (MCC) obtained from Laccosperma opacum (Rattan).
Microcrystalline cellulose (MCC) obtained from the fresh stem of Laccosperma opacum (Rattan) found in coastal region of Niger Delta forest zones of West Africa was investigated to ascertain its microbiological standard for use as a potential pharmaceutical excipient. The product, coded LO-MCC, was prepared by treating alpha–cellulose obtained from stalk of Laccosperma opacum (in pulp form) with 2.5 N hydrochloric acid followed by bleaching with 0.1N sodium hypochlorite heated to 50oC for 30 min. The product obtained was assessed for its microbiological quality and comparatively evaluated with those of fine grade MCC (Avicel PH 101) for its physicochemical, phytochemical and micromeritic properties. Result showed a whitish, tasteless, odorless crystalline MCC powder with a yield of 24.17%. Angle of repose 41±0.03o, bulk volume 82± 0.94 ml, tapped volume 52± 0.9 ml, bulk density 0.25 g/mL, tapped density 0.39 g/mL, Hausner ratio 1.59 and Carr’s compressibility index 46.7. Swelling index, moisture sorption and hydration capacities of 1.58, 0.83 and 6.46 respectively were obtained. Microbiological evaluation indicates absence of yeast, fungi as well as coliform and other objectionable bacteria. These indicate that LO- MCC complied with the minimum bioburden standard for raw materials from natural origin; its potential as disintegrant and vehicle for direct compression in tableting was evident.
Keywords: microcrystalline cellulose (MCC) Laccosperma opacum, micromeritic, Microbiological quality, Physicochemical properties, Phytochemical constituents, Physicotechnical properties