Article Sidebar
Article Details
The Momona Ethiopian Journal of Science accepts the manuscripts for consideration with the understanding that the manuscript has not been published and is not under consideration for publication elsewhere. Only original articles will be considered for publication if they have been published previously as abstracts, but not if they have been published previously as extended abstract (>1000 words). This applies to both electronic and print versions of the journal. The authors should assign copyright ownership to the Editorial Office of MEJS in the event that the manuscript is accepted for publication in the Momona Ethiopian Journal of Science. All accepted manuscripts must be accompanied by a copyright statement signed by all authors. A copy of the copyright form will be supplied along with the final reviewed version of the manuscript that is sent for final proof- reading. Authors may make multiple copies of the form if necessary and send to the Editorial Office with author’s signature(s) even individually.
All articles published by Momona Ethiopian Journal of Science (MEJS) are Open Access under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0). Under this license, authors retain ownership of the copyright for their content, and anyone can copy, distribute, or reuse articles as long as the author and original source are properly cited. In all these cases for re-use, the authors will be given proper credit to the original publication in MEJS.
Main Article Content
<em>In vitro</em> Erythrocyte Haemolysis Inhibition Properties of <em>Senna singueana</em> Extracts
Abstract
Both leaves and bark of Senna singueana (Del.) Lock(Fabaceae) are traditionally used, in some parts of Ethiopia, for the treatment of a form of skin cancer. Also the inner bark of the plant is chewed fresh to soothe stomach spasm and smoke from the wood and bark is used as smoke baths. The objective of this work was to study the antioxidant properties, of this plant using an erythrocyte haemolysis inhibition assay, because one way to justify the traditional anticancer uses can be through the concept of antioxidant effect. Crude extracts from leaves and bark of S. singueana were prepared by maceration with 80% methanol. Fractions were prepared from both leaves and bark using solvents of different polarity (diethyl ether, chloroform, and 80% methanol). The 80% methanol fraction was partitioned using ethyl acetate. These crude extracts and solvent fractions were then evaluated for their capacity to inhibit H2O2 induced erythrocyte haemolysis. Also to evaluate the presence of any intrinsic haemolysis inducing constituents such as saponins, foaming index determination and preliminary haemolysis activity determination tests were performed on extracts from the bark and leaves of the plant. The results revealed that the ethyl acetate solvent fraction from the bark of S. singueana exhibited concentration dependent erythrocyte haemolysis inhibitory activity, with an IC50 value of 233 µg/ml. However, the other solvent fractions and crude extracts did not show meaningful haemolysis inhibitory activity. The results of the foaming index determination and preliminary haemolysis activity determination tests showed the presence of saponins; but, none of the dilutions of the extracts showed any visible haemolysis activity compared to H2O2, used as a control. The results of the ethyl acetate solvent fraction from the bark of the plant could indicate the possible presence of constituents that can inhibit erythrocyte haemolysis, which in turn, could be due to lipid peroxidation inhibitory activity.
Keywords: Senna singueana, Extracts, Haemolysis Inhibition, Antioxidant
