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Genetic Manipulation of Some Cassava Varieties Using Varying Levels of Colchicine2

N. J. Amanze
P. C. Obasi


Some high yielding varieties of cassava can be very recalcitrant to flowering. As a result, become very difficult for recombination to take place. In order to address this challenge, five high yielding cassava varieties (TMS98/0505, TMS94/4479, TMS94/1632, TMS92/0057, and TMS98/0581) treated with three levels of colchicine (0, 2 and 4)ppm, were planted in a 4m x 5m plot size, at a spacing of 1mx1m in a randomized complete block design replicated three times; with the aim to improve on the architecture, flowering and capsule production at National Root Crops Research Institute, Umudike. The photosynthetic efficiency of the materials was measured using plant photosynthetic Efficient Analyzer and the result showed significant differences at (p<0.05) among varieties with TMS96/4779 as the highest (0.156). The result of the analysis of variance in the branching habits, inflorescences development and capsule production showed significant differences. There were significant differences in the number of inflorescences produced by different branch levels and the third branch (15.86cm) with the best treatment consecration of 2ppm'. The best early Variety TMS 98/0505 gave the highest branch height at each branch level with the first branch level of 6.78cm, second branch level of 14.00cm inflorescence production of (2.96) on the first branch level was found on variety TMS98/1632, while the third level of variety TMS98/0505 gave the highest number of capsules at concentration 2 ppm. This study showed that colchicine has positive significance on the induction of flowering, inflorescence and capsule production.