Persistence and Biofilm Assessment of Campylobacter Jujeni in Poultry Abattoir

  • T.V. Balogu
  • V.O. Nwaugo
  • R.A. Onyeagba
Keywords: Campylobacter jejuni, biofilm, poultry, abattoir.

Abstract

Persistence of Campylobacter sp and its biofilm forming ability was assessed in two poultry abattoirs at two weeks intervals. Average prevalence (63.75%) of Campylobacter spp. was observed on assessing a total of 160 samples collected from the surfaces of packaging table (80%), dressing table (75%), floor source (70%) and washing table (30%). Biofilm assessment formed by Campylobacter jejuni within 5-days at 370C were in decreasing order of washing table> packaging table> dressing table > floor. An average rate (19.6%) of isolates to develop biofilm observed in both sites was considered relatively low. Absorbance value (Optical Density-OD590nm) of formed biofilms ranged from 0.483 – 0.952. Wastewater from the facilities showed higher TDS (643 – 820 mgl-1), TSS (1200 – 1775 mgl-1), COD (152 – 141 mgl-1) and BOD (30.3 – 32.5mgl-1) than the WHO standards of 500 mgl-1, 100 mgl-1, 10 mgl-1 and 6 mgl-1 respectively. This is a clear indication of heavy microbial presence in the wastewater. Total bacterial count (TBC) was slightly higher in site A (4.4 x 105 CFU/ml) than site B (3.5 x 105 CFU/ml). Efficiency index ratio (≈/>1) observed in all tested drugs suggests their effectiveness in campylobacteriosis management. Decreasing drug sensitivity pedigree was observed with streptomycin> erythromycin & gentamincin > tetracycline & neomycin > penicillin> riphapicin & ampicillin > norflaxicin & cephalexin. These results of frequency and biofilm forming tendencies of Campylobacter spp. observed in this study can be of value in checkmating campybacteriosis outbreak from poultry abattoir facility.

Keywords: Campylobacter jejuni, biofilm, poultry, abattoir.

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