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Nigerian Veterinary Journal

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Comparison of agal gel precipitation test (AGPT) and enzyme linked immunosorbent assay (ELISA) in the detection of infectious bursal disease virus (IBDV) antibody in village chickens ın Oyo State, Nigeria

OA Oladele, BO Emikpe, OD Oluwayelu, OG Ohore

Abstract


The use of agar gel precipitation test (AGPT) and enzyme linked immunosorbent assay (ELISA) in assaying for the presence of infectious bursal disease (IBD) virus antibody in village chickens in Oyo State, Nigeria, was compared. Out of 400 sera subjected to ELISA, 360 (90%) samples where positive for IBD virus (Serotype 1) antibody with a titre range of 3,441 to 22,444 whereas, by the AGPT, out of 392 sera tested, 248 (63.3%) samples were positive with a titre range of 0log2 (neat) to 4log2. This study has correctly presented the seroprevalence of IBD in village chickens in this environment and has further confirmed the superior sensitivity of the ELISA technique.
Nigerian Veterinary Journal Vol.25(1) 2004: 26-29



http://dx.doi.org/10.4314/nvj.v25i1.3462
AJOL African Journals Online