Assessment of CHD-specific primers for gender determination in Red-billed Oxpeckers Buphagus erythrorhynchus

  • Desiré L Dalton
  • Antoinette Kotzé
  • Mark Howitt

Abstract

Red-billed Oxpeckers Buphagus erythrorhynchus are morphologically similar and do not display distinctive phenotypic difference between males and females. The development of DNA-based gender determination techniques constituted a breakthrough in reliable sex determination in birds. Two DNA-based methods of gender determination were evaluated to determine the preferred method for the Red-billed Oxpeckers. DNA-based gender determination of the Red-billed Oxpeckers was conducted so that specific sexes could be relocated  to new release sites within South Africa. The two primer sets used were 2550F/2718R and P2/P8. When comparing the results of the two primer sets, it was determined that 17% (n = 25) of individuals that were identified as having one sex by the 2550F/2718R primer set changed their DNA gender determination when the P2/P8 primer set was used. Based on molecular evidence and the pathology results for three recorded mortalities at the National Zoological Gardens of South Africa, it was determined that the P2/P8 primer set would be preferable to the 2550F/2718R primer set for DNA gender determination of Red-billed Oxpeckers.

OSTRICH 2010, 81(3): 251–257

Author Biographies

Desiré L Dalton
National Zoological Gardens of South Africa, PO Box 754, Pretoria 0001, South Africa;  Genetics Department, University of the Free State, PO Box 339, Bloemfontein 9300 South Africa
Antoinette Kotzé
National Zoological Gardens of South Africa, PO Box 754, Pretoria 0001, South Africa;  Genetics Department, University of the Free State, PO Box 339, Bloemfontein 9300 South Africa
Mark Howitt
National Zoological Gardens of South Africa, PO Box 754, Pretoria 0001, South Africa
Section
Articles

Journal Identifiers


eISSN: 1727-947X
print ISSN: 0030-6525