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Rickettsia rickettsii isolation from naturally infected Amblyomma parvum ticks by centrifugation in a 24-well culture plate technique

K Dzul-Rosado, G Peniche-Lara, R Tello-Martín, J Zavala-Velázquez, R de Campos Pacheco, MB Labruna, EC Sánchez, J Zavala-Castro

Abstract


Rocky Mountain spotted fever is an acute illness caused by Rickettsia rickettsii (R. rickettsii) and is  transmitted by the bite of ticks of the genera Dermacentor, Amblyomma and Rhipicephalus. The illness results  in a high mortality rate and may be easily confused with other febrile syndromes. In Yucatan State, Mexico, childhood cases with a high mortality have been reported. In this work we report the isolation of a  Mexican R. rickettsii strain from a tick egg mass using an alternative method for Rickettsia isolation with  24-well plates. We also identified a potential vector of R. rickettsii in the southeast of Mexico, which is  Amblyomma parvum.


Keywords: Amblyomma parvum, Rickettsia rickettsii, Rocky Mountain spotted fever.




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