Seroprevalence and molecular detection of Newcastle disease virus in backyard chickens in Tripoli, Libya

  • Emhemed A. Gedara
  • Hesham A. Alseed
  • Abdulwahab M. Kammon
  • Abdunaser S. Dayhum
  • Mohamed A. Benothman
  • Soleman O. Al-Garib
  • Ibrahim M. Eldaghayes
Keywords: Backyard chickens, ELISA, Newcastle disease, Prevalence, Real time PCR.

Abstract

Background: Newcastle disease (ND) is a viral disease that affecting many avian species all over the world.
Aim: ND has been successfully controlled by the vaccination of commercial poultry in Libya. However, there was a lack of information about the situation of ND in backyard chickens. Therefore, this study determined the prevalence of ND in backyard chickens in different locations of Tripoli.
Methods: A total number of 280 cloacal swabs (190 in summer and 90 in winter) and 412 sera were collected from non-vaccinated backyard chicken flocks in different geographical locations within the area of Tripoli namely Qasr Ben Ghashier, Al-Sawani, Souq Al-Gomaa, Tajourah, Ein Zara, and Janzour. Cloacal swabs and sera were tested by real time polymerase chain reaction (PCR) and ELISA, respectively.
Results: The prevalence of ND virus (NDV) infection in backyard chickens in different locations of Tripoli during summer and winter was 45% using real-time reverse transcription-PCR. Except in Qasr Ben Ghashier, the prevalence in summer season was significantly higher than in winter (X2 = 46.13, p ≥ 0.00001). ELISA test revealed 218 positive out of 412 tested samples with total prevalence of 53% across the city of Tripoli in all regions. Obviously, Qasr Ben Ghashier had significantly (X2 = 74.09, p ≥ 0.00001) the highest prevalence (82%) of NDV specific antibodies followed
by Tajourah (68%).
Conclusion: This study demonstrated the situation of ND in backyard chicken highlighting the necessity of a comprehensive vaccination plan for backyard chickens.

Keywords: Backyard chickens, ELISA, Newcastle disease, Prevalence, Real time PCR.

Published
2020-04-21
Section
Articles

Journal Identifiers


eISSN: 2218-6050
print ISSN: 2226-4485