A New Biosensor for Hydrogen Peroxide Based on Ag Colloid-modified Electrode and Azure C Mediation

  • Yan Liu
  • Ruo Yuan
  • Qunfang Li
  • Yangin Chai
  • Xia Zhong
  • Dianping Tang
  • Jianyuan Dai
Keywords: peroxidase, Ag colloid, Azure C, Hydrogen peroxide


A procedure for fabricating an enzyme electrode has been described based on the effective immobilization of horseradish peroxidase (HRP) to a nano-scaled particulate silver (nano-Ag)-modified platinum electrode with polyvinyl butyral (PVB) as the matrix. The hydrogen peroxide concentration was determined in the presence of azure C as a mediator to transfer electrons between the electrode and HRP. The HRP immobilized on colloidal Ag was stable and retained its biological activity. The biosensor displayed excellent electrocatalytic activity to the reduction of H2O2. The characteristics of the biosensor were studied by cyclic voltammetry and chronoamperometry. The effects of experimental variables such as the operating potential of the working electrode, mediator concentration, pH and temperature of the measuring solution were investigated for optimum analytical performance by using an amperometric method. Linear calibration for H2O2 was obtained in the range 2.0 x 10-6 to 6.0 x 10-3 M under the optimized conditions. The sensor was highly sensitive to H2O2 with a detection limit of 1.0 x 10-6 M and the sensor achieved 95% of the steady-state current within 5 s. The sensor exhibited high sensitivity and stability.

Keywords: Horseradish peroxidase; Ag colloid; Azure C; Hydrogen peroxide

South African Journal of Chemistry Vol.58 2005: 4-8

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eISSN: 0379-4350