Effect of hydrogen sulfide on PC12 cell injury induced by high ATP concentration

  • Kunli Yang
  • Hu Luo
  • Yajie Liu
  • Yankai Ren
  • Meixia Guo
  • Bin Wang
  • Chih-huang Yang
  • Dongliang Li
Keywords: Hydrogen sulfide, Adenosine triphosphate, Purinergic P2X7 Receptor, Cell damage, Cystathionine-β-synthase, Neural cells


Purpose: To investigate the potential protective effect of hydrogen sulfide against neural cell damage induced by a high-concentration of adenosine triphosphate (ATP).
Methods: PC12 cells were incubated with ATP in order to induce cell damage. The extracellular level of H2S and protein expression of cystathionine-β-synthase (CBS) were determined. The PC12 cells pretreated with NaHS, aminooxyacetic acid (AOAA) and KN-62, prior to further incubation with ATP, and the effect of the treatments on cell viability was investigated.
Results: High-concentration ATP induced cell death in PC12 cells, and this was accompanied by markedly increased contents of extracellular H2S and CBS expression (p < 0.05). The ATP-induced cytotoxicity was significantly compromised after pretreatment with H2S. (p < 0.05). The viability of PC12 cells pretreated with NaHS and AOAA was significantly higher than that of PC12 cells treated with ATP alone. In addition, the viability of ATP-treated PC12 cells was further markedly increased after pretreatment with NaHS and KN-62 (p < 0.05).
Conclusion: ATP induced a concentration- and time-dependent cytotoxicity in PC12 cells via the
endogenous H2S/CBS system. Supplementation with exogenous H2S mitigated the cell damage
induced by high concentration of ATP via a specific mechanism which may be specifically related to P2X7R.


Journal Identifiers

eISSN: 1596-9827
print ISSN: 1596-5996