Effects of traditional Chinese medicine on rats with Type II diabetes induced by high-fat diet and streptozotocin: A urine metabonomic study

  • H Zhao
  • Z Li
  • G Tian
  • K Gao
  • Li Zhiyong
  • B Zhao
  • J Wang
  • L Luo
  • Q Pan
  • W Zhang
  • Z Wu
  • J Chen
  • W Wang
Keywords: Type II diabetes, Urine metabonomics, Traditional Chinese Medicine, Rat Model


Background: Type II diabetes has become a serious threat to human health in recent years. Among adults above 20 years old in China, the prevalence rate of diabetes is 9.7%. Thus, it is imperative to study the mechanisms underlying type II diabetes to develop effective therapeutic treatments.
Objective: To examine metabolic changes in a rat model of type II diabetes and explore mechanisms underlying the beneficial effects of traditional Chinese medicine (TCM) in this model. 120 rats were divided into four groups, including a control group, a high-fat diet group (high-fat diet and streptozotocin injection), a TCM group (high-fat diet, streptozotocin injection, followed by TCM administration), and a rosiglitazone maleate group (high-fat diet, streptozotocin injection,
followed by rosiglitazone maleate administration). Metabolites in urine samples from 1-3 weeks (time point 1) and 4-6 weeks (time point 2) of drug administration were compared by gas chromatography-mass spectrometry (GC-MS).
Results: Our results showed that in the high-fat diet group, at time point 2, the levels of dihydroxybenzoic acid, L-ascorbic acid, D-gluconic acid, octadecanoic acid, and glutaric acid in urine were significantly higher than at time point 1. In the TCM group, at time point 2, the urine levels of L-ascorbic acid were markedly lower than at time point 1.
Conclusion: Our studies demonstrated that examining urine metabolic changes provided important insights into the mechanisms underlying type II diabetes as well as the therapeutic effects of TCM.

Key words: Type II diabetes; Urine metabonomics; Traditional Chinese Medicine; Rat Model


Journal Identifiers

eISSN: 1680-6905