Spinacia oleracea proteins with antiviral activity against tobacco mosaic virus

  • J Yang
  • G-H Jin
  • R Wang
  • Z-P Luo
  • Q-S Yin
  • L-F Jin
  • F-C Lin
Keywords: Tobacco mosaic virus, spinach, Nicotiana glutinosa, chromatography, active fraction.

Abstract

Tobacco mosaic virus (TMV) is a devastating microorganism with a global distribution and a wide host range. Protein extracts isolated from Spinacia oleracea (spinach) were examined with bioassay-guide and tested for the resistance to TMV in the Nicotiana glutinosa leaves by local lesion assay. The results show that the crude extract and the fraction of 0 to 40% ammonium sulfate precipitation could protect tobacco from TMV infection. An active fraction was further purified with cation-exchange chromatography (SP-sepharose HP) and activity screening. The inhibitory rate for TMV in vitro was up to 94.35% with 50 μg/mL of the active fraction. In addition, this active fraction could function against TMV at the infection sites on leaves of N. glutinosa. However, it had only a little inhibitory effect on TMV replication and the mechanism under the TMV-inhibitory activity of protein extract from spinach still need further investigation.

Key words: Tobacco mosaic virus, spinach, Nicotiana glutinosa, chromatography, active fraction.

Published
2014-03-25
Section
Articles

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eISSN: 1684-5315