Determination of genetic relatedness among selected rice (Oryza sativa, L.) cultivars using microsatellite markers
For plant improvement programs, genetic variation information among different cultivars is very important. Genetic variation among 26 rice (Oryza sativa, L.) accessions, consisting of 13 Iranian and 13 Malaysian cultivars was investigated using microsatellite markers distributed across the rice genome. All the 21 selected microsatellite primers were successfully amplified by polymerase chain reaction (PCR) of which 20 (95.2%) were found to be polymorphic. A total of 75 alleles were detected at 21 microsatellite loci. The allele frequencies per locus ranged from 1 in RM338 to 5 alleles in RM307, RM161, RM334 and RM271. The mean number of alleles per locus was 3.57. Amongst these microsatellite loci, the largest polymorphism information content (PIC) value was 0.74 in RM 161, while the lowest PIC value was 0.0 in RM 338. Dendrogram was constructed based on dissimilarity values, unweighted pair group with arithmetic average (UPGMA) and it separated all the cultivars into six clusters. All these polymorphisms could be further evaluated for rice marker assisted selection and developed PCR methodology would expedite screening for large numbers of rice required for association studies.
Key words: Genetic variation, genetic dissimilarity, polymorphism information content (PIC), polymerase chain reaction (PCR).