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African Journal of Biotechnology

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Study of the system of tuberous root induction in vitro from Rehmannia glutinosa

T Xue, L Guo, J-P Xue, Y-X Song, H-D Lu, A-M Zhang, W Sheng

Abstract


This study investigated the induction system of tuberous root in vitro from Rehmannia glutinosa. The roles of plant growth substance, carbohydrates, and minerals were evaluated for induction and development of tuberous root in vitro. The results show that Murashige and Skoog (MS) contributed greatly to induction of tuberous root in vitro, followed by α-naphthalene acetic acid (NAA), sucrose and 6-benzyladenine (BA). The optimal medium was ¼ MS supplemented with 1.5 mgL-1 BA, 0.15 mgL-1 NAA and 5% sucrose. In addition, paclobutrazol (PP333) and methyl jasmonate (MeJA) also played an important role in induction of tuberous root in vitro, the most appropriate concentrations were 1 mgL-1 and 10 μmolL-1 respectively.

Key words: Rehmannia glutinosa, tuberous root, tissue culture in vitro.




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