Virulence, serotype and phylogenetic groups of diarrhoeagenic Escherichia coli isolated during digestive infections in Abidjan, Côte d’Ivoire

  • Adjéhi Dadie
  • Nazaire Kouassi
  • Etienne Dako
  • Marcellin Dje
  • Mireille Dosso

Abstract

The virulence, serotype and phylogenetic traits of diarrhoeagenic Escherichia coli were detected in 502 strains isolated during digestive infections. Molecular detection of the target virulence genes, rfb gene of operon O and phylogenetic grouping genes Chua, yjaA and TSPE4.C2 was performed. Prevalence of strains harbouring virulent genes was 7.8%. The virulent genes eaeA, bfp, stx2, st1, lt, aggA, east1, ipaH, ial, cnf1 and afa were detected. EAEC (36%) and both EPEC and ATEC (25.6%) are the most detected pathovars (p<0.05). STEC (5.1%), NFEC (7.7) and DAEC (7.7) are less represented. Serogroups are overall diversified (89%), however, serogroups O157, O103 and O86, previously known to be associated with virulence were revealed. Most of the E. coli pathovars (53%) belonged to phylogenetic group A and in decreasing importance order, to D (23.5%), B1 (11.7%) and B2 (11.7%) groups. The study shows a diversified population of intestinal strains (84.6%), with a low phenotypic and phylogenetic link lower (p<0.05). Due to the great diversity of pathotypes, continuous monitoring should be implemented to identify risk factors and major pathways of contamination that help defining strategies to reduce infections associated with E. coli.

Keywords: Escherichia coli, virulence gene, serogroup, phylogenetic group, diversity

African Journal of Biotechnology, Vol. 13(9), pp. 998-1008, 26 February, 2014

Author Biographies

Adjéhi Dadie
University of Abobo-Adjamé, Faculty of food sciences and technology, Laboratory of biotechnology and food microbiology, 02 BP 801 Abidjan 02, Côte d’Ivoire
Nazaire Kouassi
National Center of Agricultural Research (CNRA), Central Laboratory of Biotechnology, 01 Bp 1740 CNRA, Abidjan, Côte d’Ivoire
Etienne Dako
University of Moncton, Laboratory of biotechnology and molecular biology, ESANEF, N.-B. E1A 3E9 Moncton, Canada
Marcellin Dje
University of Abobo-Adjamé, Faculty of food sciences and technology, Laboratory of biotechnology and food microbiology, 02 BP 801 Abidjan 02, Côte d’Ivoire
Mireille Dosso
University of Cocody, department of Medical Science; Head of Pasteur Institute, 01 Bp 490 Abidjan 01, Côte d’Ivoire
Published
2015-05-27
Section
Articles

Journal Identifiers


eISSN: 1684-5315