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African Journal of Biotechnology

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Investigation of Parietal Polysaccharides from Retama raetam Roots

H Bokhari-Taieb Brahimi, C Faugeron, K Hachem, M Kaid-Harche, V Gloaguen

Abstract


This study characterizes the cell wall hemicellulose and pectins polymers of Retama raetam. This species develops a particularly important root system and is adapted to arid areas. The cellulose, hemicelluloses and pectins were extracted. The cellulose remains the major component of the wall (27% for young roots and 80% for  adult roots), hemicelluloses (14.3% for young roots and 3.6%  for adult roots) and pectins (17.3% for young roots and 4.1% for adult roots). The monosaccharidic composition of water soluble extracts determined by gas liquid chromatography (GLC) and completed by infrared (FTIR) spectroscopy of hemicellulosic shows the presence of xylose as a major monosaccharide in the non-cellulose polysaccharides (47.8% for young roots and 59.5% for adult roots). These results indicate the presence of the homogalacturonans and rhamnogalacturonans in pectin. This study constitutes the preliminary data obtained in the biochemical analysis of the parietal compounds of the roots of a species which grows in an arid area in comparison with those of its aerial parts.

Keywords: Retama raetam, Roots, Cell Wall, Investigation, Polysaccharides, Monosaccharidic




http://dx.doi.org/10.5897/AJB2015.14754
AJOL African Journals Online