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African Journal of Biotechnology

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Analysis of phosphoric ore bacterial and eucaryal microbial diversity by single strand conformation polymorphism (SSCP) and small-subunit (SSU) sequencing

I Omri, I Manaii, Z Tlili, JJ Godon, M Hamdi

Abstract


The aim of this study was to investigate the phosphoric ore bacterial and eucaryal microbial diversity by using the single strand conformation polymorphism (SSCP) technique and small-subunit (SSU) sequencing. PCR-SSCP patterns showed a remarkably simple microbial community, mainly for bacterial community, but also for the eukaryotic community. According to the highly bacterial variable V3 region of 16S rRNA sequences, five bacterial species were identified: an unidentified species belonged to proteo gamma phylum, an unidentified species belonging to proteo delta phylum, one belonged to Moraxellaceae and two closed to Pseudomonadaceae as predominant bacteria in the mining residue. The study showed for the first time that phosphoric ore harbors major bacterial groups related to organisms having a wide range of environmentally significant functional attributes. These findings provided new opportunities into phosphoric ore microbiology that could be useful in biological system removing waste gases generated from the phosphoric industry.

Keywords: Microbial community, bacteria, archaea, eucarya, mining residue.

African Journal of Biotechnology, Vol 13(30) 3023-3029



http://dx.doi.org/10.5897/AJB2013.13090
AJOL African Journals Online