In vitro inhibition of pathogenic Verticillium dahliae, causal agent of potato wilt disease in China by Trichoderma isolates

  • C Xiaojun
  • S Wongkaew
  • Y Jie
  • Y Xuehui
  • H Haiyong
  • W Shiping
  • T Qigqun
  • W Lishuang
  • D Athinuwat
  • N Buensanteai

Abstract

Twenty (20) of Verticillium dahliae were isolated from wilted potato specimens collected from six districts in Guizhou, China. All the isolates were evaluated for pathogenicity on two potato cultivars, Favorita (susceptible) and Hui-2 (resistant) using the root dip inoculation (RDI) and microsclerotia inoculation (MI). All of the V. dahliae isolates appeared to be pathogenic on both cultivars but VGZ-HZ-4 isolate gave the highest wilt incidence comparing to the others, seconded by VGZ-SC-1 and VGZ-XW-1. Combined analysis of wilt incidence resulting from using two inoculation methods for VGZ-HZ-4 and VGZ-XW-1 isolates on the two potato cultivars showed that the MI gave a higher wilt incidence than that of the RDI and cultivar Favorita had a higher wilt incidence than that of Hui-2. These two V. dahliae isolates were further used as representative isolates for mycelial inhibition (MyI) test with 33 Trichoderma isolates under a dual culture condition on potato dextrose agar plate. The 33 Trichoderma isolates consisting of 21 isolates isolated from potato soils from seven districts of Guizhou, 11 isolates from single spore isolates of the TGZ-150 isolate preserved at Guizhou Institute of Plant Protection (GZIPP) and one isolate TGZ-OLD-81 also preserved at the GZIPP. Most of the single spore isolates and TGZ-SC-4 were found to have higher MyI efficiency than that of the rest. The results indicate that the Trichoderma isolates in this study have initial modes of action of biological control to protect potato crop against V. dahlia.

Keywords: Trichoderma, potato wilt disease, growth inhibition, Verticillium dahliae, antagonistic fungi.

African Journal of Biotechnology, Vol 13(31) 3402-3412

Author Biographies

C Xiaojun
School of Crop Production Technology, Institute of Agriculture Technology, Suranaree University of Technology Nakhon Ratchasima, 30000 Thailand
S Wongkaew
School of Crop Production Technology, Institute of Agriculture Technology, Suranaree University of Technology Nakhon Ratchasima, 30000 Thailand
Y Jie
Guizhou Institute of Plant Protection, Guizhou Academy of Agriculture Sciences Guiyang 550006, China
Y Xuehui
Guizhou Institute of Plant Protection, Guizhou Academy of Agriculture Sciences Guiyang 550006, China
H Haiyong
School of Crop Production Technology, Institute of Agriculture Technology, Suranaree University of Technology Nakhon Ratchasima, 30000 Thailand; Guizhou Institute of Plant Protection, Guizhou Academy of Agriculture Sciences Guiyang 550006, China
W Shiping
Guizhou Institute of Plant Protection, Guizhou Academy of Agriculture Sciences Guiyang 550006, China
T Qigqun
Guizhou Institute of Plant Protection, Guizhou Academy of Agriculture Sciences Guiyang 550006, China
W Lishuang
Guizhou Institute of Plant Protection, Guizhou Academy of Agriculture Sciences Guiyang 550006, China
D Athinuwat
Major of Organic Farming Management, Faculty of Science and Technology Thammasat University, Pathumthani, 12121, Thailand
N Buensanteai
School of Crop Production Technology, Institute of Agriculture Technology, Suranaree University of Technology Nakhon Ratchasima, 30000 Thailand
Published
2015-09-13
Section
Articles

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eISSN: 1684-5315