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African Journal of Biotechnology

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Polyclonal antibodies of Ganoderma boninense isolated from Malaysian oil palm for detection of basal stem rot disease

AZ Madihah, AS Idris, AR Rafidah

Abstract


Basal stem rot (BSR) disease caused by the fungus, Ganoderma boninense has become a serious threat to the oil palm industry, especially in Southeast Asia (SEA). A highly selective and sensitive diagnostic tool for BSR is extremely required for early detection, and thus, development of immunological test using enzyme-linked immunosorbent assay-polyclonal antibody (ELISA-PAb) was evaluated. Results indicate that ELISA-PAb shows recognition of Ganoderma species associated with BSR except for G. tornatum. Cross-reactivity test with fungi commonly found in oil palm plantation revealed observation of some cross-reactions with some saprophytic fungi. ELISA-PAb shows better detection as compared to cultural-based method, Ganoderma selective medium (GSM) with an improvement of 18% at nursery trial. The present study also demonstrates sensitive detection on ELISA-PAb with an increment of 30% as compared to GSM test at field trial using oil palm roots and stems. Polyclonal antibodies raised against G. boninense with positive signals was achieved, however, not specific enough for detection of BSR disease caused by Ganoderma.

Keywords: Ganoderma boninense, basal stem rot, polyclonal antibodies, enzyme-linked immunosorbent assay (ELISA).

African Journal of Biotechnology, Vol 13(31) 3455-3463



http://dx.doi.org/10.5897/AJB2013.13604
AJOL African Journals Online