Inducing and identifying artificially-induced polyploidy in bananas

  • LAS Pio
  • M Pasqual
  • S de Oliveira e Silva
  • HS Rocha
  • HM Magalhaes
  • J de Almeida Santos-Serejo

Abstract

In the present work, polyploidy was induced in the diploid banana varieties 'Malbut', 'Gold', 'Lidi', and 'Thong Dok Mak' through the use of colchicine and oryzalin, and that condition was identified through stomatal analysis, flow cytometry, and chromosome counts. Shoots produced in vitro were treated with colchicine at concentrations of 0, 2.5, 7.5 and 12.5 mM for 24 and 48 h, and with oryzalin at 0, 10, 30 and 50 mM for 4 and 7 days. Young leaves were scanned by electron microscopy to determine their stomatal areas (polar diameter × equatorial diameter) and numbers for polyploid identification by stomatal analysis. Polyploid identification by way of flow cytometry analysis used samples of young leaves that were crushed to release their nuclei, with subsequent staining with propidium iodide; ten thousand nuclei were analyzed for each sample. For cytogenetic analyses, root tips were pretreated with 0.002 M 8-HQ for 3 h, fixed in Carnoy solution for 24 h, subjected to conventional squashing techniques, and stained with 10% Giemsa. We identified four tetraploid plants and six mixoploids using these three identification techniques.

Keywords: Chromosomes duplication, Musa acuminata, tissue culture.

African Journal of Biotechnology, Vol 13(37) 3748-3758

Author Biographies

LAS Pio
UNiversidade Federal de Lavras, Departamento de Agronomia, caixa postal 3037, Box 37200-000, Lavras, Brazil
M Pasqual
Universidade Federal de Lavras, Departamento de Agronomia, caixa postal 3037, Box 37200-000, Lavras, Brazil
S de Oliveira e Silva
Emprapa Mandioca e Fruiticultura Tropical, Box 44380-000, Cruz das Almas, Brazil
HS Rocha
Emprapa Mandioca e Fruiticultura Tropical, Box 44380-000, Cruz das Almas, Brazil
HM Magalhaes
Universidade Paranaense, Box 85801-470, Umuarama Brazil
J de Almeida Santos-Serejo
Emprapa Mandioca e Fruiticultura Tropical, Box 44380-000, Cruz das Almas, Brazil
Published
2015-09-30
Section
Articles

Journal Identifiers


eISSN: 1684-5315