African Journal of Biotechnology

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A direct and sensitive method for screening fructooligosaccharides-digesting microorganisms useful in food and health science

LE Trujillo, S Granda, MI Ordonez, TI Ramos, R Avalos


Short-chain fructooligosaccharides (FOS) stimulate selectively the growth and activity of microorganisms in the colon providing positive health effects and well-being in humans and animals. The lack of accurate isolation methods, however, hampers the possibility of getting new potential fructooligosaccharides (FOS)-fermenting yeast or bacterial strains. A valuable screening procedure to visually detect bacterial and yeast strains able to ferment FOS in liquid or solid rich media supplemented with an innocuous pH indicator is described. Using this assay, 15 FOS-consuming strains isolated from different sources were successfully evaluated to prove the utility of the method. This screening procedure is a new and valuable tool in rapid large-scale detection of potential FOS fermenting-strains useful in food and health science.

Key words: FOS-fermenting microorganisms, prebiotics, probiotics, symbiotics.
AJOL African Journals Online