Short-duration exposure to 2.45 GHz microwave radiation induces DNA damage in Sprague Dawley rat’s reproductive systems

  • MR Usikalu
  • OO Obembe
  • ML Akinyemi
  • J Zhu

Abstract

The genotoxic effects of 2.45 GHz microwave (MW) radiation on the testis and ovary of Sprague Dawley rats was investigated. The animals were exposed to varying levels of specific absorption rate (SAR) of 0 (control), 0.48, 0.95, 1.43, 1.91, 2.39, 2.90, 3.40, 3.80 and 4.30 Wkg-1, for 10 min. The induction of DNA damages was assessed using DNA direct amplification of length polymorphisms (DALP) and validated with single cell gel electrophoresis (SCGE) comet assay for same cells at SAR 2.39 Wkg-1. Potential damage at the organ level was assessed by histopathological study. The results show significant differences in the Olive moment and % DNA in the blood of the exposed animals when compared with the control (p < 0.05). Hyperchromasia was observed in the ovary of the animals exposed to MW radiation. Also, there was reduction in the number of germ cells and cell disorganization in the testis of exposed group with increasing SARs. These results suggest that MW radiation has the potential to affect both male and female fertility adversely.

Keywords: 2.45 GHz microwave radiation, histopathology, DNA single strand break, ovary, testis

African Journal of Biotechnology Vol. 12(2), pp. 115-122

Author Biographies

MR Usikalu
Department of Physics, Covenant University, Ota, Ogun State, Nigeria
OO Obembe
Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria
ML Akinyemi
Department of Physics, Covenant University, Ota, Ogun State, Nigeria
J Zhu
Radiobiological Group, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China
Published
2015-11-28
Section
Articles

Journal Identifiers


eISSN: 1684-5315